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The document describes the development of a multiplex real-time PCR assay that can simultaneously detect the pathogens Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri. The assay was validated using 22 cultured amebic strains and 9 clinical specimens. The multiplex real-time PCR assay demonstrated high specificity and sensitivity down to a single ameba, with results available within 5 hours of specimen receipt. This new assay allows for rapid differential diagnosis of infections caused by t

Author

Karine Lopes

Language

EN