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Comparison of an Organic Polymeric Column and a Silica-based Reversed-phase for the Analysis of Basic Peptides by High-performance Liquid Chromatography by David V. McCalley is a Chemistry article available to read on EtoBox.
What is Comparison of an Organic Polymeric Column and a Silica-based Reversed-phase for the Analysis of Basic Peptides by High-performance Liquid Chromatography about?
The performance of a purely polymeric and a Type B silica-based C18 reversed-phase column was compared for the analysis of the basic peptide bradykinin and some analogues in order to assess the contribution of silanol interactions to peak shape. Good peak shapes were obtained for small masses of these peptides (0.1 microg or less) using acidic mobile phases on both columns; however, both showed a similar and serious deterioration in peak shape with increasing sample mass. Loss of efficiency on both columns as sample mass increased was considerably more serious when using formic acid rather than trifluoroacetic acid (TFA) as a mobile phase additive. For example, the peak capacity for a 2.5 microg load of one bradykinin on the polymeric column was reduced to only 0.38 times its value for 0.1 microg when using 0.02 M formic acid, compared with 0.77 times its value when using the same concentration of TFA. This result can be attributed to the ion pair effect of TFA and its higher ionic strength, which reduce mutual repulsion of charged peptides when held on the hydrophobic surface of the phase. Addition of salt (KCl) to the formic acid mobile phase caused dramatic increases in retentio
Who reads Comparison of an Organic Polymeric Column and a Silica-based Reversed-phase for the Analysis of Basic Peptides by High-performance Liquid Chromatography?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- David V. McCalley
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2005
- Language
- EN
- Field
- Chemistry (Physical Sciences)