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Multiple Lipid Oxidation Products in Low Density Lipoproteins Induce Interleukin-1 Beta Release from Human Blood Mononuclear Cells. by C.E. Thomas; R.L. Jackson; D.F. Ohlweiler; G. Ku is a Medicine article available to read on EtoBox.

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Oxidized low density lipoproteins (LDL) induce the release of interleukin-1 beta (IL-1 beta) from human peripheral blood mononuclear cells, a process that may contribute to atherogenesis. While 9-hydroxyoctadecadienoic acid (9-HODE) is a constituent of oxidized LDL and can by itself induce IL-1 beta release, its potency relative to oxidized LDL suggested that other components of modified LDL may also contribute to this phenomenon. In this study, LDL of varying oxidation states were prepared by altering the Cu2+ to LDL ratio and/or the length of oxidation. The oxidation status of LDL was measured as thiobarbituric acid reactive substances (TBARS), electrophoretic mobility in agarose gels, and the content of 9- and 13-HODE. High Cu2+ to LDL ratios promoted extensive TBARS formation and these LDL were the most potent activators of IL-1 beta release, although LDL with TBARS greater than 50 nmol/mg protein were cytotoxic and IL-1 beta release was diminished. An inverse correlation between HODE content and TBARS was found indicating lipid-derived aldehydes also contribute to IL-1 beta release by oxidized LDL. Accordingly, dialysis of oxidized LDL removed nearly all aldehydes and rendered

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Author

C.E. Thomas; R.L. Jackson; D.F. Ohlweiler; G. Ku

Publisher

Elsevier BV

Published

1994

Language

EN

Field

Medicine (Health Sciences)