Can I read Urinary Peptidomics Reveals Proteases Involved in Idiopathic Membranous Nephropathy on EtoBox?

Urinary Peptidomics Reveals Proteases Involved in Idiopathic Membranous Nephropathy by Baoxu Lin; Jianhua Liu; Yue Zhang; Yabin Wu; Shixiao Chen; Yibo Bai; Qiuying Liu; Xiaosong Qin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Urinary Peptidomics Reveals Proteases Involved in Idiopathic Membranous Nephropathy about?

Abstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system is implicated in various diseases. Here, we aimed to conduct urinary peptidomics to identify IMN-related proteases. Results Peptide fingerprints indicated differences in naturally produced urinary peptide components among 20 healthy individuals, 22 patients with IMN, and 15 patients with other kidney diseases. In total, 1,080 peptide-matched proteins were identified, 279 proteins differentially expressed in the urine of IMN patients were screened, and 32 proteases were predicted; 55 of the matched proteins were also differentially expressed in the kidney tissues of IMN patients, and these were mainly involved in the regulation of proteasome-, lysosome-, and actin cytoskeleton-related signaling pathways. The 32 predicted proteases showed abnormal expression in the glomeruli of IMN patients based on Gene Expression Omnibus databases. Western blot revealed abnormal expression of calpain, mat

Who reads Urinary Peptidomics Reveals Proteases Involved in Idiopathic Membranous Nephropathy?

Author

Baoxu Lin; Jianhua Liu; Yue Zhang; Yabin Wu; Shixiao Chen; Yibo Bai; Qiuying Liu; Xiaosong Qin

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)