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On the hyperthermostability of lipolytic enzymes from Thermus aquaticus YT-1: exploring their application to polymer degradation by Elena López; Beatriz Alonso; Francisco J. Deive; M. Ángeles Sanromán; María A. Longo is a Engineering article available to read on EtoBox.

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## Abstract **BACKGROUND:** The investigation of enzymes from extremophilic microorganisms has generated great scientific interest in recent decades owing to their ability to operate in harsh conditions. Since lipolytic enzymes are one of the three groups most commercially demanded, the study of novel thermostable lipolytic enzymes is of much interest. **RESULTS:** Lipolytic enzymes produced by __Thermus aquaticus__ YT1 have been investigated. Very interesting properties against thermal inactivation, with a half‐life time of 1 h at 95 °C were found. The use of compounds such as polyols is a strategy used to further improve the thermostability of these enzymes, and the modelling of the deactivation process with a series‐type equation, allows one to quantitatively ascertain the free energy of deactivation. Finally, the application to dibutyl adipate hydrolysis was investigated, as an indicator of the potential usefulness of the enzymes in polymer biodegradation. **CONCLUSION:** The crude lipolytic enzymes possess outstanding properties of thermostability (high lipolytic activity after 12 days at 80 °C, and a half‐life time of 1 h at 95 °C), and they can be significantly improved by a

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Author

Elena López; Beatriz Alonso; Francisco J. Deive; M. Ángeles Sanromán; María A. Longo

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0268-2575)

Published

2011

Language

EN

Field

Engineering (Physical Sciences)