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What is Protocol Sigama about?

The document outlines a detailed protocol for designing anti-sense oligo probes for non-coding RNA (ncRNA) and conducting Chromatin Isolation by RNA Purification (ChIRP) experiments. It includes steps for probe design, cell harvesting, cross-linking, lysis, sonication, hybridization, RNA and DNA isolation, along with necessary reagents and equipment. The methodology emphasizes the importance of using odd and even probe pools to control for non-specific signals during the experiments.

Author

wenboxu993

Language

EN