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Severe IgE-mediated anaphylaxis following consumption of fried frog legs: definition of α-parvalbumin as the allergen in cause by C. Hilger; F. Grigioni; L. Thill; L. Mertens; F. Hentges is a Medicine article available to read on EtoBox.

What is Severe IgE-mediated anaphylaxis following consumption of fried frog legs: definition of α-parvalbumin as the allergen in cause about?

**Background:** IgE‐mediated allergic reactions to bullfrog and edible frog have been reported. The implicated allergens have not been defined so far. The frog material and the patient's serum from a case of severe food‐induced anaphylaxis were used to define the implicated allergen at the protein and DNA level. **Methods:** Immunoblotting techniques and __N__‐terminal protein microsequencing were used to define the allergen recognized by the patient's serum. Back translation from the identified protein sequence was used to design degenerated primers to amplify the allergen's cDNA by polymerase chain reaction (PCR). We defined the nucleotide sequence of the allergen from the frog of Indonesian origin that was consumed by the patient, and the homologous cDNA from __Rana esculenta__. **Results:** Protein microsequencing revealed that the implicated frog allergen belonged to the parvalbumin family. cDNAs coding for α‐ and β‐parvalbumin of __R. esculenta__ and __Rana species__ were cloned. Recombinant proteins were expressed in __Escherichia coli__. The patient's serum IgE antibodies recognized parvalbumin prepared from frog muscle and recombinant α‐parvalbumin from __R. species__ but

Who reads Severe IgE-mediated anaphylaxis following consumption of fried frog legs: definition of α-parvalbumin as the allergen in cause?

Author

C. Hilger; F. Grigioni; L. Thill; L. Mertens; F. Hentges

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0105-4538)

Published

2002

Language

EN

Field

Medicine (Health Sciences)