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Amiloride Derivatives Enhance Insulin Release in Pancreatic Islets from Diabetic Mice by Subhadra C Gunawardana; W Steven Head; David W Piston is a Medicine article available to read on EtoBox.

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## Abstract ## Background Amiloride derivatives, commonly used for their diuretic and antihypertensive properties, can also cause a sustained but reversible decrease of intracellular pH (pH~i~). Using dimethyl amiloride (DMA) on normal rodent pancreatic islets, we previously demonstrated the critical influence of islet pH~i~ on insulin secretion. Nutrient-stimulated insulin secretion (NSIS) requires a specific pH~i~-range, and is dramatically enhanced by forced intracellular acidification with DMA. Furthermore, DMA can enable certain non-secretagogues to stimulate insulin secretion, and induce time-dependent potentiation (TDP) of insulin release in mouse islets where this function is normally absent. The present study was performed to determine whether pH~i~-manipulation could correct the secretory defect in islets isolated from mice with type 2 diabetes. ## Methods Using two mouse models of type 2 diabetes, we compared a) pHi-regulation, and b) NSIS with and without treatment with amiloride derivatives, in islets isolated from diabetic mice and wild type mice. ## Results A majority of the islets from the diabetic mice showed a slightly elevated basal pH~i~ and/or poor recovery fro

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Author

Subhadra C Gunawardana; W Steven Head; David W Piston

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1472-6823)

Published

2005

Language

EN

Field

Medicine (Health Sciences)