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Alteration of Calcium Signalling in Cardiomyocyte Induced by Simulated Microgravity and Hypergravity by Caizhi Liu; Guohui Zhong; Yuezhang Zhou; Yuchen Yang; Yingjun Tan; Yuheng Li; Xingcheng Gao; Weijia Sun; Jianwei Li; Xiaoyan Jin; Dengchao Cao; Xinxin Yuan; Zizhong Liu; Shuai Liang; Youyou Li; Ruikai Du; Yinlong Zhao; Jianqi Xue; Dingsheng Zhao; Jinping Song; Shukuan Ling; Yingxian Li is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Alteration of Calcium Signalling in Cardiomyocyte Induced by Simulated Microgravity and Hypergravity about?
## Abstract ## Objectives Cardiac Ca^2+^ signalling plays an essential role in regulating excitation‐contraction coupling and cardiac remodelling. However, the response of cardiomyocytes to simulated microgravity and hypergravity and the effects on Ca^2+^ signalling remain unknown. Here, we elucidate the mechanisms underlying the proliferation and remodelling of HL‐1 cardiomyocytes subjected to rotation‐simulated microgravity and 4G hypergravity. ## Materials and Methods The cardiomyocyte cell line HL‐1 was used in this study. A clinostat and centrifuge were used to study the effects of microgravity and hypergravity, respectively, on cells. Calcium signalling was detected with laser scanning confocal microscopy. Protein and mRNA levels were detected by Western blotting and real‐time PCR, respectively. Wheat germ agglutinin (WGA) staining was used to analyse cell size. ## Results Our data showed that spontaneous calcium oscillations and cytosolic calcium concentration are both increased in HL‐1 cells after simulated microgravity and 4G hypergravity. Increased cytosolic calcium leads to activation of calmodulin‐dependent protein kinase II/histone deacetylase 4 (CaMKII/HDAC4) signalli
Who reads Alteration of Calcium Signalling in Cardiomyocyte Induced by Simulated Microgravity and Hypergravity?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Caizhi Liu; Guohui Zhong; Yuezhang Zhou; Yuchen Yang; Yingjun Tan; Yuheng Li; Xingcheng Gao; Weijia Sun; Jianwei Li; Xiaoyan Jin; Dengchao Cao; Xinxin Yuan; Zizhong Liu; Shuai Liang; Youyou Li; Ruikai Du; Yinlong Zhao; Jianqi Xue; Dingsheng Zhao; Jinping Song; Shukuan Ling; Yingxian Li
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7722)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)