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Can I read The isolation, characterization and sequence of two divergent ?-tubulin genes from soybean (Glycine max L.) on EtoBox?
The isolation, characterization and sequence of two divergent ?-tubulin genes from soybean (Glycine max L.) by Mark J. Guiltinan; Din Pow Ma; Richard F. Barker; Mauricio M. Bustos; Richard J. Cyr; Ramin Yadegari; Donald E. Fosket is a Agricultural and Biological Sciences article available to read on EtoBox.
What is The isolation, characterization and sequence of two divergent ?-tubulin genes from soybean (Glycine max L.) about?
Two divergent ~-tubulin genes (designated Sf3-1 and S~3-2) were isolated by screening a soybean genomic library with a Chlamydomonas reinhardtii/3-tubulin cDNA probe. Restriction fragment analysis of the clones recovered, and of soybean genomic DNA, indicated that these represent two unique classes of structurally different /~-tubulin genes in the soybean genome. However, it is possible that unidentified members of these classes or additional highly divergent classes of/3-tubulin genes (thus far undetected) exist in the soybean genome. The S~3-1 and St3-2 genomic clones were sequenced, revealing that both are potentially functional genes which would encode/3-tubulins of 445 and 449 amino acids, respectively. A comparison of their derived amino acid sequences with B-tubulins from several organisms showed that they are most homologous to Chlamydomonas B-tubulin (85 -87%), with lesser degrees of homology to/3-tubulins of vertebrate species (79-83%), Trypanosoma brucei (80-81%) and Saccharomyees eerevisiae (66-68%). The amino acid sequences of SB-1 and S/3-2 are as divergent from each other as they are from the Chlamydomonas B-tubulin. The amino acids at the diverged positions in S~-2
Who reads The isolation, characterization and sequence of two divergent ?-tubulin genes from soybean (Glycine max L.)?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Mark J. Guiltinan; Din Pow Ma; Richard F. Barker; Mauricio M. Bustos; Richard J. Cyr; Ramin Yadegari; Donald E. Fosket
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)
- Published
- 1987
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)