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Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA) by Wim Fremout; Stepanka Kuckova; Michaela Crhova; Jana Sanyova; Steven Saverwyns; Radovan Hynek; Milan Kodicek; Peter Vandenabeele; Luc Moens is a Chemistry article available to read on EtoBox.

What is Classification of protein binders in artist's paints by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry: an evaluation of principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA) about?

Proteomics techniques are increasingly applied for the identification of protein binders in historical paints. The complex nature of paint samples, with different kinds of pigments mixed into, and degradation by long term exposure to light, humidity and temperature variations, requires solid analysis and interpretation methods. In this study matrix‐assisted laser desorption/ionisation time‐of‐flight (MALDI‐TOF) mass spectra of tryptic‐digested paint replicas are subjected to principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA) in order to distinguish proteinaceous binders based on animal glues, egg white, egg yolk and milk casein from each other. The most meaningful peptide peaks for a given protein class will be determined, and if possible, annotated with their corresponding amino acid sequence. The methodology was subsequently applied on egg temperas, as well as on animal glues from different species. In the latter small differences in the MALDI‐TOF mass spectra can allow the determination of a mammal or sturgeon origin of the glue. Finally, paint samples from the 16^th^ century altarpiece of St Margaret of Antioch (Mlynica, Slovakia) were an

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Author

Wim Fremout; Stepanka Kuckova; Michaela Crhova; Jana Sanyova; Steven Saverwyns; Radovan Hynek; Milan Kodicek; Peter Vandenabeele; Luc Moens

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2011

Language

EN

Field

Chemistry (Physical Sciences)