About this document
DNA Sequencing Techniques Overview by Marcelino Calata is a document available to read on EtoBox.
This document summarizes three methods of DNA sequencing: direct sequencing, pyrosequencing, and bisulfite sequencing. Direct sequencing involves manually determining the order of nucleotides in a DNA polymer through chemical or chain termination methods. Pyrosequencing sequences DNA by detecting light produced during DNA synthesis. Bisulfite sequencing is modified chain termination sequencing used to detect methylated cytosine residues by treating DNA with sodium bisulfite and PCR amplification.
- Author
- Marcelino Calata
- Language
- EN