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Separation and purification of anthocyanins from Roselle by macroporous resins 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemicals and materials 2 2.2 Pretreatment of macroporous resins 2 2.3 Determination of anthocyanin content 2 2.4 Optimization of extraction solvent for Roselle anthocyanins 2 2.5 Preparation of Roselle extract for resin adsorption 2 2.6 Static adsorption kinetics 2 2.7 Adsorption isotherms 2 2.8 Dynamic adsorption 3 2.9 Desorption test 3 2.10 Purification of anthocyanins from Roselle extract through DM21 resin 3 2.11 Statistical analysis 3 3 Results and discussion 3 3.1 Extraction of Roselle anthocyanins with different solvents 3 3.2 Static adsorption kinetics comparison between the six resins 4 3.3 Adsorption isotherm 4 3.4 Dynamic adsorption analysis 5 3.5 Desorption and regeneration study 6 3.6 Purification of anthocyanins from Roselle by DM21 resin 6 4 Conclusion 6 CRediT authorship contribution statement 7 Declaration of competing interest 7 Acknowledgements 7 Appendix A Supplementary data 7 References 7

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Language

EN

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nonfiction