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Molecular Imaging With Targeted Perfluorocarbon Nanoparticles: Quantification of the Concentration Dependence of Contrast Enhancement for Binding to Sparse Cellular Epitopes by Jon N. Marsh; Kathryn C. Partlow; Dana R. Abendschein; Michael J. Scott; Gregory M. Lanza; Samuel A. Wickline is a Medicine article available to read on EtoBox.
What is Molecular Imaging With Targeted Perfluorocarbon Nanoparticles: Quantification of the Concentration Dependence of Contrast Enhancement for Binding to Sparse Cellular Epitopes about?
Targeted, liquid perfluorocarbon nanoparticles are effective agents for acoustic contrast enhancement of abundant cellular epitopes (e.g., fibrin in thrombi) and for lower prevalence binding sites, such as integrins associated with tumor neovasculature. In this study, we sought to delineate the quantitative relationship between the extent of contrast enhancement of targeted surfaces and the density (and concentration) of bound perfluorocarbon (PFC) nanoparticles. Two dramatically different substrates were utilized for targeting. In one set of experiments, the surfaces of smooth, flat, avidin-coated agar disks were exposed to biotinylated nanoparticles to yield a thin layer of targeted contrast. For the second set of measurements, we targeted PFC nanoparticles applied in thicker layers to cultured smooth muscle cells expressing the transmembrane glycoprotein "tissue factor" at the cell surface. An acoustic microscope was used to characterize reflectivity for all samples as a function of bound PFC (determined via gas chromatography). We utilized a formulation of low-scattering nanoparticles having oil-based cores to compete against high-scattering PFC nanoparticles for binding, to el
Who reads Molecular Imaging With Targeted Perfluorocarbon Nanoparticles: Quantification of the Concentration Dependence of Contrast Enhancement for Binding to Sparse Cellular Epitopes?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Jon N. Marsh; Kathryn C. Partlow; Dana R. Abendschein; Michael J. Scott; Gregory M. Lanza; Samuel A. Wickline
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-5629)
- Published
- 2007
- Language
- EN
- Field
- Medicine (Health Sciences)