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Can I read Electropolymerized-molecularly imprinted polymers (E-MIPS) as sensing elements for the detection of dengue infection on EtoBox?
Electropolymerized-molecularly imprinted polymers (E-MIPS) as sensing elements for the detection of dengue infection by Clarisse E. Buensuceso; Brylee David B. Tiu; Luke P. Lee; Portia Mahal G. Sabido; Guillermo M. Nuesca; Eugene B. Caldona; Florian R. del Mundo; Rigoberto C. Advincula is a Chemistry article available to read on EtoBox.
What is Electropolymerized-molecularly imprinted polymers (E-MIPS) as sensing elements for the detection of dengue infection about?
A straightforward in situ detection method for dengue infection was demonstrated through the molecular imprinting of a dengue nonstructural protein 1 (NS1) epitope into an electropolymerized molecularly imprinted polyterthiophene (E-MIP) film sensor. The key enabling step in the sensor fabrication is based on an epitope imprinting strategy, in which short peptide sequences derived from the original target molecules were employed as the main template for detection and analysis. The formation of the E-MIP sensor films was facilitated using cyclic voltammetry (CV) and monitored in situ by electrochemical quartz crystal microbalance (EC-QCM). Surface properties were analyzed using different techniques including atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and polarization modulation-infrared reflection-adsorption (PM-IRRAS). The standard calibration curve (R = 0.9830) was generated for the detection of the epitope, Ac-VHTWTE-QYKFQ-NH 2 , with a linear range of 0.2 to 30 μg/mL and detection limit of 0.073 μg/mL. A separate calibration curve (R = 0.9786) was obtained using spiked buffered solutions of dengue NS1 protein, which resulted in a linear range of 0.2 t
Who reads Electropolymerized-molecularly imprinted polymers (E-MIPS) as sensing elements for the detection of dengue infection?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Clarisse E. Buensuceso; Brylee David B. Tiu; Luke P. Lee; Portia Mahal G. Sabido; Guillermo M. Nuesca; Eugene B. Caldona; Florian R. del Mundo; Rigoberto C. Advincula
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Chemistry (Physical Sciences)