Can I read Water Droplets and Ice Deposits in Leaf Intercellular Spaces: Redistribution of Water During Cryofixation for Scanning Electron Microscopy on EtoBox?
Water Droplets and Ice Deposits in Leaf Intercellular Spaces: Redistribution of Water During Cryofixation for Scanning Electron Microscopy by C. E. Jeffree; N. D. Read; J. A. C. Smith; J. E. Dale is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Water Droplets and Ice Deposits in Leaf Intercellular Spaces: Redistribution of Water During Cryofixation for Scanning Electron Microscopy about?
An experimental study is described of the formation of extracellular deposits on the surfaces of cells in freeze-fractured, frozen-hydrated primary leaves of Phaseolus vulgaris examined by low-temperature scanning electron microscopy. The deposits, observed under a range of experimental conditions, consisted of (a) droplets with diameters of 1.5 to 3.0 μm, (b) droplets with diameters of 10 to 30 μm, (c) crystals with diameters of 1.0 to 6.0 μm, and (d) granules with diameters up to 0.15 μm. The types of deposit were influenced by specimen cooling rate, and their distribution was influenced by the direction of the thermal gradient during cooling. All deposits were predominantly water ice. The quantities of deposited water (up to 4.0% of the leaf water content) increased as the cooling rate was reduced. It is concluded that the ice deposits were primarily artefacts of cryofixation and do not represent the location of water in vivo, as recently suggested. We propose that the deposits arose in four main ways: (1) displacement of water from underlying cells by a pressure wave resulting from the volume increase of intracellular water as it freezes, (2) evaporation of water from warmer ce
Who reads Water Droplets and Ice Deposits in Leaf Intercellular Spaces: Redistribution of Water During Cryofixation for Scanning Electron Microscopy?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- C. E. Jeffree; N. D. Read; J. A. C. Smith; J. E. Dale
- Publisher
- Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)
- Published
- 1987
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)