Can I read On-line Coupling of Solid-phase Extraction with Mass Spectrometry for the Analysis of Biological Samples on EtoBox?

On-line Coupling of Solid-phase Extraction with Mass Spectrometry for the Analysis of Biological Samples by C.H.P Bruins; C.M Jeronimus-Stratingh; K Ensing; W.D van Dongen; G.J de Jong is a Chemistry article available to read on EtoBox.

What is On-line Coupling of Solid-phase Extraction with Mass Spectrometry for the Analysis of Biological Samples about?

The potential of the direct coupling of solid-phase extraction (SPE) with mass spectrometry (MS) for the analysis of biological samples is demonstrated. For SPE a cartridge exchanger is used and the eluate is directly introduced into the mass spectrometer. This system has been investigated for the determination of clenbuterol in urine. With mixed-mode cartridges, a considerable ion suppression has been obtained. The mass spectrum at the elution time of clenbuterol is dominated by that of creatinine and adduct formation of clenbuterol and creatinine has been observed. The whole procedure including injection of 1 ml urine, washing and desorption has been developed with cartridges containing 8-mm C -bonded silica. If only a single 18 MS step is used, the selectivity and, therefore, the sensitivity are insufficient. The detection limit is about 100 ng / ml. However, with atmospheric pressure chemical ionisation and the tandem MS mode the detection limit has been decreased to about 2 ng / ml and the ion suppression is only about 10%. For the electrospray ionisation the detection limit is about 10-times higher and the ion suppression is less favourable. The repeatability for the SPE-MS-M

Who reads On-line Coupling of Solid-phase Extraction with Mass Spectrometry for the Analysis of Biological Samples?

Author

C.H.P Bruins; C.M Jeronimus-Stratingh; K Ensing; W.D van Dongen; G.J de Jong

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

1999

Language

EN

Field

Chemistry (Physical Sciences)