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Controlled release of sex steroids through osmotic pump alters brain GnRH1 and catecholaminergic system dimorphically in the catfish, Clarias gariepinus by Mamta, Sajwan-Khatri; Sudhakumari, Chenichery; Kagawa, Hirohiko; Dutta-Gupta, Aparna; Senthilkumaran, Balasubramanian is a Neuroscience article available to read on EtoBox.
What is Controlled release of sex steroids through osmotic pump alters brain GnRH1 and catecholaminergic system dimorphically in the catfish, Clarias gariepinus about?
The present study aimed to evaluate osmotic pump-mediated controlled release of estrogen in males and androgen in females to analyze the impact on gonadotropin-releasing hormone (GnRH1), catecholamines (CAs) and other associated genes in the catfish, Clarias gariepinus. During pre-spawning phase, catfish were separately implanted osmotic pumps loaded with 17β-estradiol (E 2 ) in males and 17α-methyltestosterone (MT) in females at a dose of 10 μg/100 μl or saline (100 μl) controls into both sexes to release for 21 days and all fishes were maintained as per the duration. Further, GnRH1 expression levels were analysed in the discrete regions of brain after E 2 and MT treatments in male and female catfish, respectively using qPCR which revealed that GnRH1 expression was significantly higher in E 2 treated male as compared to the control. On the other hand, GnRH1 expression was lower in MT treated female when compared to the control in the discrete regions of brain. In addition, certain brain and monoaminergic system related genes showed a differential response. Catfish GnRH1 could be localized in preoptic area-hypothalamus (POA-HYP) that correlated with the expression profile in the di
Who reads Controlled release of sex steroids through osmotic pump alters brain GnRH1 and catecholaminergic system dimorphically in the catfish, Clarias gariepinus?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Mamta, Sajwan-Khatri; Sudhakumari, Chenichery; Kagawa, Hirohiko; Dutta-Gupta, Aparna; Senthilkumaran, Balasubramanian
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0361-9230)
- Published
- 2020
- Language
- EN
- Field
- Neuroscience (Life Sciences)