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Elucidation of Metabolism in Hybridoma Cells Grown in Fed‐batch Culture by Genome‐scale Modeling by Suresh Selvarasu; Victor V.T. Wong; Iftekhar A. Karimi; Dong‐Yup Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Genome‐scale modeling of mouse hybridoma cells producing monoclonal antibodies (mAb) was performed to elucidate their physiological and metabolic states during fed‐batch cell culture. Initially, feed media nutrients were monitored to identify key components among carbon sources and amino acids with significant impact on the desired outcome, for example, cell growth and antibody production. The monitored profiles indicated rapid assimilation of glucose and glutamine during the exponential growth phase. Significant increase in mAb concentration was also observed when glutamine concentration was controlled at 0.5 mM as a feeding strategy. Based on the reconstructed genome‐scale metabolic network of mouse hybridoma cells and fed‐batch profiles, flux analysis was then implemented to investigate the cellular behavior and changes in internal fluxes during the cell culture. The simulated profile of the cell growth was consistent with experimentally measured specific growth rate. The in silico simulation results indicated (i) predominant utilization of glycolytic pathway for ATP production, (ii) importance of pyruvate node in metabolic shifting, and (iii) characteristic pattern

Who reads Elucidation of Metabolism in Hybridoma Cells Grown in Fed‐batch Culture by Genome‐scale Modeling?

Author

Suresh Selvarasu; Victor V.T. Wong; Iftekhar A. Karimi; Dong‐Yup Lee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)