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Cell monolayer deformation microscopy reveals mechanical fragility of cell monolayers following EMT by Amy A. Sutton; Clayton W. Molter; Ali Amini; Johanan Idicula; Max Furman; Pouria Tirgar; Yuanyuan Tao; Ajinkya Ghagre; Newsha Koushki; Adele Khavari; Allen J. Ehrlicher is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Cell monolayer deformation microscopy reveals mechanical fragility of cell monolayers following EMT about?
Tissue and cell mechanics are crucial factors in maintaining homeostasis and in development, with aberrant mechanics contributing to many diseases. During the epithelial-to-mesenchymal transition (EMT), a highly conserved cellular program in organismal development and cancer metastasis, cells gain the ability to detach from their original location and autonomously migrate. While a great deal of biochemical and biophysical changes at the single-cell level have been revealed, how the physical properties of multicellular assemblies change during EMT, and how this may affect disease progression, is unknown. Here we introduce cell monolayer deformation microscopy (CMDM), a new methodology to measure the planar mechanical properties of cell monolayers by locally applying strain and measuring their resistance to deformation. We employ this new method to characterize epithelial multicellular mechanics at early and late stages of EMT, finding the epithelial monolayers to be relatively compliant, ductile, and mechanically homogeneous. By comparison, the transformed mesenchymal monolayers, while much stiffer, were also more brittle, mechanically heterogeneous, displayed more viscoelastic cree
Who reads Cell monolayer deformation microscopy reveals mechanical fragility of cell monolayers following EMT?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Amy A. Sutton; Clayton W. Molter; Ali Amini; Johanan Idicula; Max Furman; Pouria Tirgar; Yuanyuan Tao; Ajinkya Ghagre; Newsha Koushki; Adele Khavari; Allen J. Ehrlicher
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)