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Kinetics of Osteoprogenitor Proliferation and Osteoblast Differentiation in Vitro by Luc Malaval; Fina Liu; Patricia Roche; Jane E. Aubin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Kinetics of Osteoprogenitor Proliferation and Osteoblast Differentiation in Vitro about?

Fetal rat calvaria cells plated at very low density generate discrete colonies, some of which are bone colonies (nodules) from individual osteoprogenitors that divide and differentiate. We have analyzed the relationship between cell proliferation and acquisition of tissue-specific differentiation markers in bone colonies followed individually from the original single cell to the fully mineralized state. The size distribution of fully formed nodules is unimodal, suggesting that the coupling between proliferation and differentiation of osteoprogenitor cells is governed by a stochastic element, but distributed around an optimum, corresponding to the peak colony size/division potential. Kinetic analysis of colony growth showed that osteoprogenitors undergo 9-10 population doublings before the appearance of the first morphologically differentiated osteoblasts in the developing colony. Double immunolabeling showed that these proliferating cells express a gradient of bone markers, from proliferative alkaline phosphatase-negative cells at the periphery of colonies, to postmitotic, osteocalcin-producing osteoblasts at the centers. An inverse relationship exists between cell division and exp

Who reads Kinetics of Osteoprogenitor Proliferation and Osteoblast Differentiation in Vitro?

Author

Luc Malaval; Fina Liu; Patricia Roche; Jane E. Aubin

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)