Can I read Brain-wide Microglia Replacement Using a Nonconditioning Strategy Ameliorates Pathology in Mouse Models of Neurological Disorders on EtoBox?

Brain-wide Microglia Replacement Using a Nonconditioning Strategy Ameliorates Pathology in Mouse Models of Neurological Disorders by Dadian Chen1†, Chen Wang2†, Xi Chen3†, Jiayu Li1†, Shuai Chen4, Yanzhong Lingting Li1, Mengling Zou1, Xin Li1, Xiaohua Huang5, Yun-wu Zhang1, Yingjuonghua Zheng1*, Xiao-Fen Chen1,7*, Jie Zhang1*, Li Zhong1,7,8* is a book available to read on EtoBox.

What is Brain-wide Microglia Replacement Using a Nonconditioning Strategy Ameliorates Pathology in Mouse Models of Neurological Disorders about?

Growing genetic and pathological evidence has identified microglial dysfunction as a key contributor to the pathogenesis and progression of various neurological disorders, positioning microglia replacement as a promising therapeutic strategy. Traditional bone marrow transplantation (BMT) methods for replenishing brain microglia have limitations, including low efficiency and the potential for brain injury because of preconditioning regimens, such as irradiation or chemotherapy. Moreover, BM-derived cells that migrate to the brain do not recapitulate the phenotypic and functional properties of resident microglia. Here, we present a microglia transplantation strategy devoid of any conditioning, termed “tricyclic microglial depletion for transplantation” (TCMDT). This approach leverages three cycles of microglial depletion using the colony stimulating factor 1 receptor (CSF1R) inhibitor PLX3397, creating an optimal window for efficient engraftment of exogenous microglia. Transplantation of primary cultured microglia by TCMDT successfully restored the identity and functions of endogenous microglia. To evaluate the therapeutic potential of TCMDT, we applied this strategy to two distin

Author

Dadian Chen1†, Chen Wang2†, Xi Chen3†, Jiayu Li1†, Shuai Chen4, Yanzhong Lingting Li1, Mengling Zou1, Xin Li1, Xiaohua Huang5, Yun-wu Zhang1, Yingjuonghua Zheng1*, Xiao-Fen Chen1,7*, Jie Zhang1*, Li Zhong1,7,8*

Published

2025

Language

EN