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Impact of dissolved oxygen concentration on acetate accumulation and physiology of E. coli BL21, evaluating transcription levels of key genes at different dissolved oxygen conditions by Je-Nie Phue; Joseph Shiloach is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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High density growth of Escherichia coli especially in large bioreactors may temporarily expose the cells to oxygen limitation as a result of a local inadequate oxygen supply or intermittently high concentrations of cells and nutrients. Although short, these periods can potentially alter bacterial metabolism, affecting both growth and recombinant proteins production capability, and thus lowering process productivity. When E. coli B (BL21), a lower acetate producing strain, was grown aerobically on high glucose, acetate accumulation was found to be inversely correlated to the dissolved oxygen (DO) levels, reaching 10 g/L at 1%, 4 g/L at 6%, and zero at 30% DO concentration at stationary growth phase. Time-course transcription analysis of several genes involved in glucose and acetate metabolism indicated that the enhanced acetate production at lower DO levels is the result of altered transcription of several key genes. These genes are: the acetate producing gene (poxB), the glyoxylate shunt gene (aceA), the acetate uptake gene (acs), the gluconeogensis and anaplerotic pathways genes, (pckA, ppsA, ppc, and sfcA), the TCA cycle gene (gltA), and the sigma factors 70 and S (rpoD and rpoS)

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Author

Je-Nie Phue; Joseph Shiloach

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 1096-7176)

Published

2005

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)