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Two replication initiation sites on R-plasmid DNA by Jorge H. Crosa; Linda K. Luttropp; Fred Heffron; Stanley Falkow is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Two replication initiation sites on R-plasmid DNA about?

Replicating DNA molecules of a deletion mutant of the conjugative R-plasmid R 6 K are cleaved at a single site by the EcoRI restriction endonuclease. Electron microscope examination and measurements of the EcoRI treated replicative intermediate molecules indicate that replication can be initiated at two sites on the plasmid DNA molecule. The two sites are located at about 23 and 39% of total length, respectively, from the EcoRI cleavage site. About 5% of the replicating molecules use both replication initiation sites simultaneously.

Who reads Two replication initiation sites on R-plasmid DNA?

Author

Jorge H. Crosa; Linda K. Luttropp; Fred Heffron; Stanley Falkow

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1975

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)