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Can I read Directed Evolution and Biophysical Characterization of a Full-length, Soluble, Human Caveolin-1 Variant on EtoBox?
Directed Evolution and Biophysical Characterization of a Full-length, Soluble, Human Caveolin-1 Variant by Smith, Joshua N.; Edgar, Joshua M.; Balk, J. Mark; Iftikhar, Mariam; Fong, Jessica C.; Olsen, Tivoli J.; Fishman, Dmitry A.; Majumdar, Sudipta; Weiss, Gregory A. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Directed Evolution and Biophysical Characterization of a Full-length, Soluble, Human Caveolin-1 Variant about?
Protein engineering by directed evolution can alter proteins' structures, properties, and functions. However, membrane proteins, despite their importance to living organisms, remain relatively unexplored as targets for protein engineering and directed evolution. This gap in capabilities likely results from the tendency of membrane proteins to aggregate and fail to overexpress in bacteria cells. For example, the membrane protein caveolin-1 has been implicated in many cell signaling pathways and diseases, yet the full-length protein is too aggregation-prone for detailed mutagenesis, directed evolution, and biophysical characterization. Using a phage-displayed library of full-length caveolin-1 variants, directed evolution with alternating subtractive and functional selections isolated a full-length, soluble variant, termed cav, for expression in E. coli. Cav folds correctly and binds to its known protein ligands HIV gp41, the catalytic domain of cAMP-dependent protein kinase A, and the polymerase I and transcript release factor. As expected, cav does not bind off-target proteins. Cellular studies show that cav retains the parent protein's ability to localize at the cellular membrane.
Who reads Directed Evolution and Biophysical Characterization of a Full-length, Soluble, Human Caveolin-1 Variant?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Smith, Joshua N.; Edgar, Joshua M.; Balk, J. Mark; Iftikhar, Mariam; Fong, Jessica C.; Olsen, Tivoli J.; Fishman, Dmitry A.; Majumdar, Sudipta; Weiss, Gregory A.
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1570-9639)
- Published
- 2018
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)