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Can I read Flow Cytometric Analysis of Apoptosis in Cryoconserved Chicken Primordial Germ Cells on EtoBox?
Flow Cytometric Analysis of Apoptosis in Cryoconserved Chicken Primordial Germ Cells by Dorota Sawicka; Luiza Chojnacka-Puchta; Marcin Zielinski; Grazyna Plucienniczak; Andrzej Plucienniczak; Marek Bednarczyk is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Flow Cytometric Analysis of Apoptosis in Cryoconserved Chicken Primordial Germ Cells about?
Our research aimed to compare the effects of four cryoprotectants and four slow freezing programs on the viability and apoptosis of primordial germ cells (PGCs) in vitro. PGCs were collected from chicken embryonic blood at Hamburger and Hamilton (HH) stages 14-16 and purified by Percoll density gradient centrifugation and then subjected to cryopreservation. We applied microscopy to determine the survival of PGCs after trypan blue staining and flow cytometry to examine apoptosis and viability after annexin V kit staining. We also examined the functionality of cryopreserved PGCs in vivo. Significant differences in viability of PGCs determined via microscopy and flow cytometry were observed. The most unfavorable combination for slow freezing PGCs was program 3 and MIX H (10% DMSO and 5% glycerol in Hank's solution supplemented with 10% FBS) as the cryoprotectant (48.43 and 15.37% live and early apoptotic PGCs, respectively). The highest average percentage of live PGCs (93.1%) and the lowest percentage of early apoptotic PGCs (6.5%) were achieved by slow freezing PGCs in the presence of DMSO F (10% DMSO in FBS) via program 1. Therefore, this method was chosen for the in vivo test. Cryo
Who reads Flow Cytometric Analysis of Apoptosis in Cryoconserved Chicken Primordial Germ Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Dorota Sawicka; Luiza Chojnacka-Puchta; Marcin Zielinski; Grazyna Plucienniczak; Andrzej Plucienniczak; Marek Bednarczyk
- Publisher
- Walter de Gruyter GmbH
- Published
- 2015
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)