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What is Long-Term Cultivation of HCEC about?
The study details a method for isolating and long-term cultivating human corneal endothelial cells (HCEC) using enzymatic treatment with collagenase and trypsin. It demonstrates that HCEC can be maintained in culture for over 20 generations without senescence, utilizing a selective medium to inhibit fibroblast growth. The inclusion of extracellular matrix components and fibroblast growth factor enhances cell proliferation and supports monolayer formation.
- Author
- lauribto
- Language
- EN