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Differential protein expression by ** Porphyromonas gingivalis ** in response to secreted epithelial cell components by Yi Zhang; Tiansong Wang; Weibin Chen; Özlem Yilmaz; Yoonsuk Park; Il‐Young Jung; Murray Hackett; Richard J. Lamont is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Differential protein expression by ** Porphyromonas gingivalis ** in response to secreted epithelial cell components about?
## Abstract The human oral pathogen __Porphyromonas gingivalis__ colonizes the gingival crevice and invades gingival epithelial cells. Multidimensional capillary high‐performance liquid chromatography coupled with tandem mass spectrometry and two‐dimensional gel electrophoresis were used to analyze the proteome of __P. gingivalis__ as it adapts to a set of experimental conditions designed to reflect important features of an epithelial cell environment. 1014 proteins (46% of the total theoretical proteome) were identified in four independent analyses; 479 of these proteins showed evidence of differential expression after exposure of __P. gingivalis__ to either conditioned epithelial cell growth medium or control conditions: __i.e.__, they were only detected under one set of conditions. Moreover, 276 genes annotated as hypothetical were found to encode expressed proteins. Among the proteins up‐regulated in the presence of epithelial cell components were a homolog of the internalin proteins of __Listeria monocytogenes__ and subunits of the ATP‐dependent Clp protease complex. Insertional inactivation of __clpP__, encoding the Clp proteolytic subunit, resulted in approximately a 50% red
Who reads Differential protein expression by ** Porphyromonas gingivalis ** in response to secreted epithelial cell components?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yi Zhang; Tiansong Wang; Weibin Chen; Özlem Yilmaz; Yoonsuk Park; Il‐Young Jung; Murray Hackett; Richard J. Lamont
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)