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Intrinsic differences in cisplatin sensitivity of head and neck cancer cell lines: Correlation to lysosomal pH by Cathrine Nilsson; Karin Roberg; Roland C. Grafström; Karin Öllinger is a Medicine article available to read on EtoBox.

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## Abstract ## Background Cisplatin treatment is beneficial for approximately 20% of patients with head and neck squamous cell carcinoma (HNSCC). Tools to predict the clinical outcome and evaluate intrinsic cisplatin sensitivity are, therefore, required. ## Methods Cisplatin sensitivity, lysosomal pH, and cell death pathway was studied in 5 HNSCC lines and compared with normal oral keratinocytes. ## Results We identified a linear relationship between lysosomal pH and cisplatin sensitivity. Reduced lysosomal acidification was correlated to decreased expression of the V~0~V~1~‐ATPase B2 subunit, which is part of the lysosomal acidifying complex. Cisplatin caused apoptosis accompanied by lysosomal membrane permeabilization, and inhibition of lysosomal proteases (cathepsins) partly prevented cell death. ## Conclusion Cisplatin‐induced apoptosis of HNSCC is more efficient in cell lines with low lysosomal pH and is mediated by the release of lysosomal content. Lysosomal pH and expression of V~0~V~1~‐ATPase subunits are possible future markers of intrinsic cisplatin sensitivity. © 2009 Wiley Periodicals, Inc. Head Neck, 2010

Who reads Intrinsic differences in cisplatin sensitivity of head and neck cancer cell lines: Correlation to lysosomal pH?

Author

Cathrine Nilsson; Karin Roberg; Roland C. Grafström; Karin Öllinger

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 1043-3074)

Published

2010

Language

EN

Field

Medicine (Health Sciences)