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1,25‐dihydroxy‐vitamin‐D~3~ enhances antiproliferative effect and transcription of TGF‐β1 on human keratinocytes in culture by Hee-Jong Kim; Neamatallah Abdelkader; Marion Katz; John A. McLane is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Both TGF‐β and 1,25‐dihydroxy‐vitamin‐D~3~ (1,25(OH)~2~D~3~) have been reported to decrease the proliferation of normal human keratinocytes. The effect and expression of TGF‐β in keratinocytes treated with 1,25(OH)~2~D~3~ was investigated. Human keratinocytes were grown in the presence of various concentrations of TGF‐β and/or 1,25(OH)~2~D~3~ prior to enumeration. TGF‐β, alone, has a half maximal dose of inhibition (ED~50~) of approximately 750 pg/ml after seven days in culture in Keratinocyte Growth Medium (KGM^®^; Clonetics) supplemented with 1.5 mM calcium. When 1,25(OH)~2~D~3~ (10^−7^M) was also added to cultures with various concentrations of TGF‐β, the ED~50~ shifted an average of 2‐fold less. The presence of TGF‐β (10 pg/ml) augmented the potency of 1,25(OH)~2~D~3~ by at least 10‐fold. In keratinocyte cultures, the antiproliferative effect of the two compounds together is synergistic. In keratinocytes grown for 1 week in the presence of 1,25(OH)~2~D~3~ at 10^−6^M, the TGF‐β1 message increased approximately 5‐fold. An increase is detected within 2 hours of exposure to 1,25(OH)~2~D~3~. There was only a 50% increase in the levels of TGF‐β2 and no detection of TGF‐β3
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- Author
- Hee-Jong Kim; Neamatallah Abdelkader; Marion Katz; John A. McLane
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 1992
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)