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Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli by Rani Sauriasari; Da-Hong Wang; Yoko Takemura; Ken Tsutsui; Noriyoshi Masuoka; Kuniaki Sano; Masako Horita; Bing-Ling Wang; Keiki Ogino is a Environmental Science article available to read on EtoBox.
What is Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli about?
Lawsone is an active naphthoquinone derivative isolated from henna (Lawsonia inermis L.), a widely used hair dye. Previous study on the toxicity of lawsone remains unclear since the involvement of oxidative stress and the kind of ROS (reactive oxygen species) involved have not been fully resolved yet. This present study reports the cytotoxic effects of lawsone and henna. We carried out CAT assay (a zone of inhibition test of bacterial growth and colony-forming efficiency test of transformant Escherichia coli strains that express mammalian catalase gene derived from normal catalase mice (Cs a ) and catalase-deficient mutant mice (Cs b )), Ames mutagenicity assay and H 2 O 2 generation assay. Lawsone generated H 2 O 2 slightly in phosphate buffer system and was not mutagenic in Ames assay using TA 98, TA 100 and TA 102, both in the absence and presence of metabolic activation. Lawsone exposure inhibited the growth of both Cs a and Cs b strains in a dose-dependent manner. Mean zone diameter for Cs a was 9.75 ± 0.96 mm and 12.75 ± 1.5 mm for Cs b . Natural henna leaves did not show toxic effects, whereas two out of four samples of marketed henna products were shown toxicity effects. Ca
Who reads Cytotoxicity of lawsone and cytoprotective activity of antioxidants in catalase mutant Escherichia coli?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Rani Sauriasari; Da-Hong Wang; Yoko Takemura; Ken Tsutsui; Noriyoshi Masuoka; Kuniaki Sano; Masako Horita; Bing-Ling Wang; Keiki Ogino
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0300-483X)
- Published
- 2007
- Language
- EN
- Field
- Environmental Science (Life Sciences)