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Sugarcane Mosaic Virus in Plantlets Regenerated from Diseased Leaf Tissue by J. E. Irvine; G. T. A. Benda is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Sugarcane Mosaic Virus in Plantlets Regenerated from Diseased Leaf Tissue about?

Plantlets produced from sugarcane leaf tissue were examined to determine the effect of propagation on the frequency of occurrence of sugarcane mosaic virus (SCMV). Explants from immature leaf tissues of the sugarcane variety CP 72-356 (Saccharum interspecific hybrid), healthy or SCMV-infected, were cultured on Murashige-Skoog medium to which a combination of cytokinin and auxin had been added. Plantlets developed on healthy and infected leaf tissue within 6 weeks. The juice from plantlets was assayed for SCMV on Rio sorghum (Sorghum bicolor (L.) Moench, var. Rio) seedlings and on sugarcane varieties CP 31-294 and CO 31-588 for SCMV-strain identification. Results indicated that SCMV strain H was transmitted from the donor tissue to the regenerated plantlets. Observation on plantlets reared in the greenhouse showed that 23% had symptoms of SCMV. In a second replicated experiment, the leaf tissue from plants of POJ 234 free of mosaic or infected with SCMV strain A, B, D, H, or I was cultured. Each of the five strains was transmitted from donor to plantlet as indicated by assays on sorghum and sugarcane varieties. From 11 to 88% of the plantlets had mosaic symptoms, depending on the st

Who reads Sugarcane Mosaic Virus in Plantlets Regenerated from Diseased Leaf Tissue?

Author

J. E. Irvine; G. T. A. Benda

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0167-6857)

Published

1985

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)