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Can I read Monitoring of the internalization of neuropeptide Y on neuroblastoma cell line SK‐N‐MC on EtoBox?
Monitoring of the internalization of neuropeptide Y on neuroblastoma cell line SK‐N‐MC by Marlies Fabry; Michael Langer; Barbara Rothen‐Rutishauser; Heidi Wunderli‐Allenspach; Hartwig Höcker; Annette G. Beck‐Sickinger is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Monitoring of the internalization of neuropeptide Y on neuroblastoma cell line SK‐N‐MC about?
Neuropeptide Y (NPY) is an important neuromodulator in the central and peripheral nervous system. The peptide acts through different NPY receptor subtypes (Y1–Y5, y6) that belong to the family of G protein‐coupled receptors. In general, cellular responses to prolonged exposure to agonists of G protein‐coupled receptors are attenuated, often through internalization of the receptors and their bound ligands. In this study, a fluorescent labeled NPY derivative was synthesized and characterized to investigate the internalization of NPY in the human neuroblastoma cell line SK‐N‐MC. Internalization was proven by binding experiments and subsequent acidic washing as well as by direct visualization by means of confocal laser scanning microscopy. Approximately 20–30% of the fluorescent labeled NPY and a tritium‐marked NPY were resistant to acid removal of cell surface‐bound ligands indicating internalization. Extracellular fluorescent labeled NPY was found to be distributed heterogeneously in a clustered pattern, which suggests that the ligand‐receptor complex is collected in pits and caveolae followed by endocytosis.
Who reads Monitoring of the internalization of neuropeptide Y on neuroblastoma cell line SK‐N‐MC?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Marlies Fabry; Michael Langer; Barbara Rothen‐Rutishauser; Heidi Wunderli‐Allenspach; Hartwig Höcker; Annette G. Beck‐Sickinger
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)