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Profilin in Phaseolus vulgaris is encoded by two genes (only one expressed in root nodules) but multiple isoforms are generated in vivo by phosphorylation on tyrosine residues by Gabriel Guillén; Víctor Valdés‐López; Raúl Noguez; Juan Olivares; Luis Carlos Rodríguez‐Zapata; Héctor Pérez; Luis Vidali; Marco A. Villanueva; Federico Sánchez is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Profilin in Phaseolus vulgaris is encoded by two genes (only one expressed in root nodules) but multiple isoforms are generated in vivo by phosphorylation on tyrosine residues about?

## Summary Actin‐binding proteins such as profilins participate in the restructuration of the actin cytoskeleton in plant cells. Profilins are ubiquitous actin‐, polyproline‐, and inositol phospholipid‐binding proteins, which in plants are encoded by multigene families. By 2D‐PAGE and immunoblotting, we detected as much as five profilin isoforms in crude extracts from nodules of __Phaseolus vulgaris__. However, by immunoprecipitation and gel electrophoresis of __in vitro__ translation products from nodule RNA, only the most basic isoform of those found in nodule extracts, was detected. Furthermore, a bean profilin cDNA probe hybridised to genomic DNA digested with different restriction enzymes, showed either a single or two bands. These data indicate that profilin in __P. vulgaris__ is encoded by only two genes. In root nodules only one gene is expressed, and a single profilin transcript gives rise to multiple profilin isoforms by post‐translational modifications of the protein. By __in vivo__^32^P‐labelling and immunoprecipitation with both, antiprofilin and antiphosphotyrosine‐specific antibodies, we found that profilin is phosphorylated on tyrosine residues. Since chemical (TLC)

Who reads Profilin in Phaseolus vulgaris is encoded by two genes (only one expressed in root nodules) but multiple isoforms are generated in vivo by phosphorylation on tyrosine residues?

Author

Gabriel Guillén; Víctor Valdés‐López; Raúl Noguez; Juan Olivares; Luis Carlos Rodríguez‐Zapata; Héctor Pérez; Luis Vidali; Marco A. Villanueva; Federico Sánchez

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1999

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)