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Can I read Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C on EtoBox?
Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C by Gustavo C. Bressan; Eduardo C. Moraes; Adriana O. Manfiolli; Tais M. Kuniyoshi; Dario O. Passos; Marcelo D. Gomes; Jörg Kobarg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C about?
## Abstract The human SFRS9/SRp30c belongs to the SR family of splicing regulators. Despite evidence that members of this protein family may be targeted by arginine methylation, this has yet to be experimentally addressed. In this study, we found that SFRS9 is a target for PRMT1-mediated arginine methylation in vitro, and that it is immunoprecipitated from HEK-293 lysates by antibodies that recognize both mono- and dimethylated arginines. We further observed that upon treatment with the methylation inhibitor Adox, the fluorescent EGFP-SFRS9 re-localizes to dot-like structures in the cell nucleus. In subsequent confocal analyses, we found that EGFP-SFRS9 localizes to nucleoli in Adox-treated cells. Our findings indicate the importance of arginine methylation for the subnuclear localization of SFRS9.
Who reads Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gustavo C. Bressan; Eduardo C. Moraes; Adriana O. Manfiolli; Tais M. Kuniyoshi; Dario O. Passos; Marcelo D. Gomes; Jörg Kobarg
- Publisher
- SP Versita; Springer (Biomed Central Ltd.); BioMed Central Ltd.; Springer Science and Business Media LLC; Walter de Gruyter GmbH (ISSN 1425-8153)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)