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What is HPLC Protocol for Analyzing Polyamines about?

This document describes a protocol for analyzing polyamines in biological samples using HPLC. The method involves pre-column derivatization of polyamines with o-phthalaldehyde and N-acetyl-L-cysteine to form fluorescent derivatives, which are separated via reversed-phase HPLC and detected fluorometrically. The protocol optimizes sample processing and deproteinization for accurate polyamine analysis in various small-volume biological samples. The method provides sensitive, reproducible, and automated analysi

Author

FRANCIS NDOUR

Language

EN