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Cordycepin reprogramming lipid metabolism to block metastasis and EMT via ERO1A/mTOR/SREBP1 axis in cholangiocarcinoma 1 1 Introduction 1 2 Materials and methods 2 2.1 Cell culture and reagents 2 2.2 Online database analysis 2 2.3 Lentivirus transfection and stable cell clone establishment 2 2.4 siRNA transfection 2 2.5 MTT assay 2 2.6 Colony formation assay 2 2.7 5-Ethyl-2′-deoxyuridine (EdU) incorporation assay 3 2.8 Wound healing assay 3 2.9 Migration assay 3 2.10 Fatty acid metabolism detection 3 2.11 Western blotting 3 2.12 In vivo metastasis assays 3 2.13 Immunohistochemistry (IHC) staining and H&E staining 3 2.14 Statistics 3 3 Results 3 3.1 Cordycepin inhibits CCA cell proliferation, migration and EMT in vitro 3 3.2 Cordycepin regulates CCA cells lipid metabolism via AKT/mTOR signaling pathways 5 3.3 ERO1A is highly expressed in CCA 5 3.4 ERO1A promotes migration and EMT procession through regulating the AKT/mTOR/SREBP1 axis in CCA cells 5 3.5 Cordycepin inhibits CCA cells migration, EMT process and lipid metabolism by regulating ERO1A 5 3.6 Cordycepin inhibits the biological process of CCA through ERO1A/mTOR/SREBP1 axis 8 3.7 Silencing SREBP1 suppresses CCA cells prolifera

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nonfiction