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Broadly cross‐reactive mimotope of hypervariable region 1 of hepatitis C virus derived from DNA shuffling and screened by phage display library by Xin‐Xin Zhang; Qiang Deng; Shen‐Ying Zhang; Jing Liu; Qian Cai; Zhi‐Meng Lu; Yuan Wang is a Medicine article available to read on EtoBox.

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## Abstract The hypervariable region 1 (HVR1) is the target of neutralizing antibodies but with isolate specificity. The aim of this study was to obtain immunogenic mimotopes of HVR1, which can react broadly with different HVR1 antibodies and could be one of the candidate immunogens in an effective vaccine against HCV. Thirty‐one HVR1 cDNA fragments were digested by DNase I into a pool of random fragments and reassembled by repeated cycles of annealing in the presence of DNA polymerase to their original size. The shuffled HVR1 was then inserted into the gene III phagemid vector pCANTAB‐5E and displayed on the surface of the phage. Eight individual phages were selected after four rounds of biopanning against anti‐HVR1. ELISA was carried out on immobilized purified phages, respectively, to detect their reactivity with a panel of sera. DNA sequences of the inserts were analyzed and compared with the consensus sequences defined by Puntoriero et al. [(1998) EMBO J 17:3521–3533]. The reactivity of the eight selected clones to the 30 sera were from 53.3 to 80%. Among these, phage 13 (ETYVSGGSAARNAYGLTSLFTVGPAQK, aa 384–410) reacted most broadly. None of the selected sequences encoded for

Who reads Broadly cross‐reactive mimotope of hypervariable region 1 of hepatitis C virus derived from DNA shuffling and screened by phage display library?

Author

Xin‐Xin Zhang; Qiang Deng; Shen‐Ying Zhang; Jing Liu; Qian Cai; Zhi‐Meng Lu; Yuan Wang

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0146-6615)

Published

2003

Language

EN

Field

Medicine (Health Sciences)