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Current Protocols in Molecular Biology by Frederick M. Ausubel; Roger Brent; Robert E. Kingston; David Moore; J. G. Seidman; Kevin Struhl is a nonfiction available to read on EtoBox.

What is Current Protocols in Molecular Biology about?

cover ......Page 1 Contents ......Page 13 CHAPTER 1 -- Escherichia coli, Plasmids, and Bacteriophages......Page 26 E coli ......Page 0 Media Preparation and Bacteriological Tools......Page 29 Growth in Liquid Media......Page 36 Growth on Solid Media......Page 38 Selected Topics from Classical Bacterial Genetics ......Page 44 VECTORS DERIVED FROM PLASMIDS -- Introduction to Plasmid Biology......Page 58 Minipreps of Plasmid DNA......Page 75 PREPARATION OF CRUDE LYSATE BY ALKALINE LYSIS......Page 85 PREPARATION OF CRUDE LYSATE BY THE BOILING METHOD......Page 88 PREPARATION OF CRUDE LYSATE BY TRITON LYSIS......Page 89 PURIFICATION OF PLASMID DNA BY CsCl/ETHIDIUM BROMIDE EQUILIBRIUM CENTRIFUGATION......Page 90 PLASMID DNA PURIFICATION BY PEG PRECIPITATION......Page 93 PLASMID DNA PURIFICATION BY ANION-EXCHANGE OR SIZE EXCLUSION CHROMATOGRAPHY......Page 94 TRANSFORMATION USING CALCIUM CHLORIDE......Page 101 ONE-STEP PREPARATION AND TRANSFORMATION OF COMPETENT CELLS......Page 103 HIGH-EFFICIENCY TRANSFORMATION BY ELECTROPORATION......Page 104 DIRECT ELECTROPORETIC TRANSFER OF PLASMID DNA FROM YEAST INTO E. COLI......Page 106 Introduction to Lambda Phages......Page 111 Lambda as a Clon

Who reads Current Protocols in Molecular Biology?

Author

Frederick M. Ausubel; Roger Brent; Robert E. Kingston; David Moore; J. G. Seidman; Kevin Struhl

Publisher

John Wiley & Sons, Incorporated

Published

2003

Language

EN

ISBN

9780471625940

Category

nonfiction

Subjects

Science, Technology, Biology