Can I read A digital assay for programmed death-ligand 1 (22C3) quantification combined with immune cell recognition algorithms in non-small cell lung cancer on EtoBox?

A digital assay for programmed death-ligand 1 (22C3) quantification combined with immune cell recognition algorithms in non-small cell lung cancer by Paces, Will (author);Ergon, Elliott (author);Bueche, Elizabeth (author);Young, G. Dave (author);Adisetiyo, Vitria (author);Luengo, Cris (author);James, Meredith (author);Caldwell, Charles (author);Miller, Dannah (author);Wambaugh, Morgan (author);Metcalf, Geoffrey (author);Gianani, Roberto (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A digital assay for programmed death-ligand 1 (22C3) quantification combined with immune cell recognition algorithms in non-small cell lung cancer about?

PD-L1 (22C3) checkpoint inhibitor therapy represents a mainstay of modern cancer immunotherapy for non-small cell lung cancer (NSCLC). In vitro diagnostic (IVD) PD-L1 antibody staining is widely used to predict clinical intervention efficacy. However, pathologist interpretation of this assay is cumbersome and variable, resulting in poor positive predictive value concerning patient therapy response. To address this, we developed a digital assay (DA) termed Tissue Insight (TI) 22C3 NSCLC, for the quantification of PD-L1 in NSCLC tissues, including digital recognition of macrophages and lymphocytes. We completed clinical validation of this digital image analysis solution in 66 NSCLC patient samples, followed by concordance studies (comparison of PD-L1 manual and digital scores) in an additional 99 patient samples. We then combined this DA with three distinct immune cell recognition algorithms for detecting tissue macrophages, alveolar macrophages, and lymphocytes to aid in sample interpretation. Our PD-L1 (22C3) DA was successfully validated and had a scoring agreement (digital to manual) higher than the inter-pathologist scoring. Furthermore, the number of algorithm-identified immune

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Author

Paces, Will (author);Ergon, Elliott (author);Bueche, Elizabeth (author);Young, G. Dave (author);Adisetiyo, Vitria (author);Luengo, Cris (author);James, Meredith (author);Caldwell, Charles (author);Miller, Dannah (author);Wambaugh, Morgan (author);Metcalf, Geoffrey (author);Gianani, Roberto (author)

Publisher

Springer Science and Business Media LLC

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)