About this document
Plasmid Size Analysis via Gel Electrophoresis by Walwin Hare is a document available to read on EtoBox.
The document summarizes an experiment aimed at isolating and analyzing recombinant plasmids from transformed bacterial cells using a rapid miniprep procedure. Agarose gel electrophoresis revealed that two topological forms of DNA were present - linear and supercoiled. Comparison of the sizes of bands to expected sizes of the vector (pTrc99A) and recombinant plasmid (pTrc99A cysB) indicated that the extracted plasmid was the vector, as its observed size of approximately 3200 bp matched the expected size, whe
- Author
- Walwin Hare
- Language
- EN