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Endo‐β‐1,4‐glucanases Are Involved in Peach Fruit Growth and Ripening, and Regulated by Ethylene by Claudio Bonghi; Luca Ferrarese; Benedetto Ruperti; Pietro Tonutti; Angelo Ramina is a Agricultural and Biological Sciences article available to read on EtoBox.

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During peach (__Prunus persica__[L.] Batsch) fruit development and ripening the cell wall undergoes several structural and biochemical changes driven by several hydrolases. Among these, the endo‐β‐1,4‐D‐glucanase (EGase, EC 3.2.1.4), or cellulase, may play a crucial role. Involvement of EGase throughout development and ripening of the fruit of cv. Redhaven was assessed by monitoring enzyme activity, specific polypeptide accumulation and gene transcription. During the four stages of growth EGase activity was high during S1 and in the early S2, declined during S3, and increased with the onset of ripening (S4). Two isoforms with isolectric points of 6.5 and 9.5 were identified. The pI 6.5 EGase was the only form present during the early stages of growth. whereas the pI 9.5 EGase was most abundant during ripening. The same isoforms were present in leaf and fruit abscission zones. The antibody raised against the pI 9.5 EGase, purified from leaf abscission zones, cross‐reacted with a protein of 54 kDa. A cDNA clone of 753 bp encoding peach EGase was obtained by RT‐PCR. EGase transcripts, detectable only after amplification of total RNA by RT‐PCR, were observed during S1, and at the precl

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Author

Claudio Bonghi; Luca Ferrarese; Benedetto Ruperti; Pietro Tonutti; Angelo Ramina

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0031-9317)

Published

1998

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)