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Can I read Hybridization method for quantitation of replicating polyoma DNA sequences on EtoBox?

Hybridization method for quantitation of replicating polyoma DNA sequences by Unni Spaeren; Waldemar Waldeck; Rolf Eliasson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Hybridization method for quantitation of replicating polyoma DNA sequences about?

Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybridization experiments to localize the isotope in different parts of the polyoma genome after in vitro incorporation of labeled deoxyribonucleoside triphosphates into the DNA.

Who reads Hybridization method for quantitation of replicating polyoma DNA sequences?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Unni Spaeren; Waldemar Waldeck; Rolf Eliasson
Publisher
Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
Published
1979
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

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