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Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.) by Monique Burrus; Christel Chanabe; Gilbert Alibert; Dennis Bidney is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.) about?

Sunflower hypocotyl protoplasts (Helianthus annuus L.) from 5 PIONEER genotypes (PT024, SMF3, EMIL, HA300\*PT024, VK5F) and 1 public line (RHa 274) formed colonies at frequencies of up to 60% when plated in 0.25ml agarose beads in a modified L4 medium (Lenée and Chupeau 1986) containing 3mg/l NAA, 1mg/l BA and 0.1mg/l 2,4-D, and 1000mg/l casamino acids. Protoplast-derived colonies grew slowly into calli. Organogenesis was obtained from callus of PT024 on a MS medium containing NAA and BA at 1mg/l and GA at 0.1mg/l. Freshly excised shoots were induced to root by an IAA treatment. Regenerated plants were transferred to the greenhouse and seed was harvested within 7 months of the initial protoplast isolation.

Who reads Regeneration of fertile plants from protoplasts of sunflower (Helianthus annuus L.)?

Author

Monique Burrus; Christel Chanabe; Gilbert Alibert; Dennis Bidney

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0721-7714)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)