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A standardized method for the quantification of polysaccharides: An example of polysaccharides from Tremella fuciformis 1 1 Introduction 1 2 Materials and methods 2 2.1 Plant materials 2 2.2 Reagents 2 2.3 Preparation of TFPS from Tremella fuciformis 2 2.4 Determination of TFPS with phenol – sulfuric acid (PSA) method 2 2.4.1 Preparation of color reagents 2 2.4.2 Acid hydrolysis 2 2.4.3 Color reaction temperature 2 2.4.4 Enzyme interference 2 2.5 Enzyme calibration standard curve 3 2.6 TFPS sample storage conditions 3 2.7 Data analysis 3 3 Results and discussion 3 3.1 Current issues in the determination of phenol sulfate method 3 3.2 Key parameters affecting the accurate determination 4 3.2.1 Acid hydrolysis pretreatment steps 4 3.2.2 Accurate and reliable temperature control 4 3.2.3 Interferences caused by enzymatic extractions 5 3.3 Calibration and quantitative analysis of TFPS 5 3.4 Storage conditions of polysaccharide samples 7 3.5 Final optimized procedure for accurate determination and quantification of polysaccharides 7 4 Conclusion 7 CRediT authorship contribution statement 8 Declaration of competing interest 8 Data availability 8 Acknowledgments 8 Appendix A Supplementary

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UND

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nonfiction