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Human papillomavirus DNA and p53 status in penile carcinomas by José E. Levi; Paula Rahal; Álvaro S. Sarkis; Luisa L. Villa is a Medicine article available to read on EtoBox.

What is Human papillomavirus DNA and p53 status in penile carcinomas about?

Our study aimed at evaluating the presence of human papillomavirus (HPV) DNA in a series of 84 paraffinembedded (PET) penile carcinomas. We have also investigated the presence of p53 mutations in these tumors by immunohistochemistry (IHC), single-stranded conformational polymorphism (SSCP) and DNA sequencing. Tissues were submitted to amplification of a 268 bp fragment from the ␤-globin gene and a fragment of the E6 gene of HPV types 6, 11, 16 and 18. Twenty samples (18 fixed in Bouin's solution and 2 in buffered formalin) were found inadequate and were excluded from the analysis. In the remaining 64 tumors, HPV DNA was found in 26% of the samples. The prevalence of HPV in fresh samples of the same tumors was 56%. The most prevalent type was HPV 16 in both fresh samples and PET. Isotopic in situ hybridization was performed in all PET samples, but only 2 cases were positive, 1 for HPV 16 and 1 for HPV 18. Immunohistochemistry with anti-p53 pAb1801 antibody showed a positive nuclear reaction over more than 5% of tumor cells in 26% of the cases. SSCP of exons 5-8 of the p53 gene was performed on 9 HPV-positive and 12 HPV-negative specimens. Abnormal mobility was found in 26% of the tu

Who reads Human papillomavirus DNA and p53 status in penile carcinomas?

Author

José E. Levi; Paula Rahal; Álvaro S. Sarkis; Luisa L. Villa

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1998

Language

EN

Field

Medicine (Health Sciences)