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Recognition of Human Hemoglobin with Macromolecularly Imprinted Polymeric Nanoparticles Using Non‐covalent Interactions by Gülgün Aylaz; Müge Andaç; Adil Denizli; Memed Duman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Recognition of Human Hemoglobin with Macromolecularly Imprinted Polymeric Nanoparticles Using Non‐covalent Interactions about?

## Abstract Hemoglobin (Hb) is the most abundant protein in the blood. It is vital for the living as oxygen carriers. Some of the very pure Hb‐containing biological fluids are currently under clinical trial. However, the removal and purification of Hb from the blood are quite difficult, especially when it is at a low concentration level. In this study, the molecularly imprinted polymeric nanoparticles (MIPNs) were prepared using N‐methacryloyl‐histidine methyl ester (MAH) by mini‐emulsion polymerization technique for specific binding of human hemoglobin (HHb). MIPNs in monosize form have a size of 152 ± 4 nm. They also have a high binding capacity (32.33 mg/g) of HHb. MIPNs retain 84% of the re‐binding capacity for HHb after 10 cycles. The nanoparticles have 16 and 5 times higher binding capacity of HHb, respectively, in the presence of bovine serum albumin and lysozyme. Thanks to their high binding capacity and selectivity, MIPNs will allow them to be detected selectively for different target molecules. According to molecular docking, the main binding forces depend on hydrogen bonds and Van der Waals forces in the interaction within 5 Å around MAH molecule are observed through the

Who reads Recognition of Human Hemoglobin with Macromolecularly Imprinted Polymeric Nanoparticles Using Non‐covalent Interactions?

Author

Gülgün Aylaz; Müge Andaç; Adil Denizli; Memed Duman

Publisher

Wiley

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)