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Mesenchymal Stromal Cells from Primary Osteosarcoma Are Non-malignant and Strikingly Similar to Their Bone Marrow Counterparts by Jan C. Brune; Ariane Tormin; Maria C. Johansson; Pehr Rissler; Otte Brosjö; Richard Löfvenberg; Fredrik Vult von Steyern; Fredrik Mertens; Anders Rydholm; Stefan Scheding is a Medicine article available to read on EtoBox.

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Mesenchymal stromal cells (MSC) are multipotent cells that can be isolated from a number of human tissues. In cancer, MSC have been implicated with tumor growth, invasion, metastasis, drug resistance and were even suggested as possible tumorinitiating cells in osteosarcoma (OS). However, MSC from OS and their possible tumor origin have not yet been thoroughly investigated. Therefore, primary OS mesenchymal progenitors and OS-derived MSC were studied. OS samples contained very high frequencies of mesenchymal progenitor cells as measured by the colony-forming unit fibroblast (CFU-F) assay (median: 1,117 colonies per 10 5 cells, range: 133-3,000, n 5 6). This is considerably higher compared to other human tissues such as normal bone marrow (BM) (1.3 6 0.2 colonies per 10 5 cells, n 5 8). OS-derived MSC (OS-MSC) showed normal MSC morphology and expressed the typical MSC surface marker profile (CD105/CD73/CD90/CD44/HLA-classI/CD166 positive, CD45/CD34/CD14/CD19/HLA-DR/CD31 negative). Furthermore, all OS-MSC samples could be differentiated into the osteogenic lineage, and all but one sample into adipocytes and chondrocytes. Genetic analysis of OS-MSC as well as OS-derived spheres showed

Who reads Mesenchymal Stromal Cells from Primary Osteosarcoma Are Non-malignant and Strikingly Similar to Their Bone Marrow Counterparts?

Author

Jan C. Brune; Ariane Tormin; Maria C. Johansson; Pehr Rissler; Otte Brosjö; Richard Löfvenberg; Fredrik Vult von Steyern; Fredrik Mertens; Anders Rydholm; Stefan Scheding

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2010

Language

EN

Field

Medicine (Health Sciences)