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Functional Analysis of Rhomboid Proteases during Toxoplasma Invasion by Bang Shen; Jeffrey S. Buguliskis; Tobie D. Lee; L. David Sibley is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## ABSTRACT Host cell invasion by __Toxoplasma gondii__ and other apicomplexan parasites requires transmembrane adhesins that mediate binding to receptors on the substrate and host cell to facilitate motility and invasion. Rhomboid proteases (ROMs) are thought to cleave adhesins within their transmembrane segments, thus allowing the parasite to disengage from receptors and completely enter the host cell. To examine the specific roles of individual ROMs during invasion, we generated single, double, and triple knockouts for the three ROMs expressed in __T. gondii__ tachyzoites. Analysis of these mutants demonstrated that ROM4 is the primary protease involved in adhesin processing and host cell invasion, whereas ROM1 or ROM5 plays negligible roles in these processes. Deletion of ROM4 blocked the shedding of adhesins such as MIC2 (microneme protein 2), causing them to accumulate on the surface of extracellular parasites. Increased surface adhesins led to nonproductive attachment, altered gliding motility, impaired moving junction formation, and reduced invasion efficiency. Despite the importance of ROM4 for efficient invasion, mutants lacking all three ROMs were viable and MIC2 was sti

Who reads Functional Analysis of Rhomboid Proteases during Toxoplasma Invasion?

Author

Bang Shen; Jeffrey S. Buguliskis; Tobie D. Lee; L. David Sibley

Publisher

American Society for Microbiology

Published

2014

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)