About this Environmental Science article
Biodegradation characteristics of lignin in pulping wastewater by the thermophilic Serratia sp. AXJ-M: Performance, genetic background, metabolic pathway and toxicity assessment by Xuejiao An; Yi Cheng; Hailian Zang; Chunyan Li is a Environmental Science article available to read on EtoBox.
The key to the efficient removal of pulping wastewater lies in the effective degradation of lignin at high temperature. There is thus an urgent need to seek effective eco-environmental techniques to overcome this environmental limit for lignin degradation. The soil isolate thermophilic Serratia sp. AXJ-M efficiently metabolizes lignin. Nevertheless, the underlying comprehensive molecular mechanism of lignin degradation by thermophilic AXJ-M is poorly understood. Here, strain AXJ-M showed excellent degradation ability toward diverse lignin-related aromatic compounds. Functional genome analysis and RNA-Seq disclosed several traits which in joint consideration suggest a high efficiency of AXJ-M representative to the lignin degradation and environmental adaptation. Multiomics analyses combined with GC–MS revealed seven potential lignin biodegradation pathways. DyP was predicted to be involved in the breakdown of the β-O-4 ether bond, Cα-Cβ bond and Cα oxidation of lignin by prokaryotic expression and gene knockout and complementation. Molecular docking deepens the understanding of the interaction between DyP and lignin. Toxicity assessment experiments clearly indicated that AXJ-M signi
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Xuejiao An; Yi Cheng; Hailian Zang; Chunyan Li
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Environmental Science (Physical Sciences)